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البيانات الشخصية للإستاذ الدكتور/

حمدى عبدالعظيم حسن على

الاســــم

حمدى عبدالعظيم حسن على

الوظيفة

أستاذ دكتور بجامعة مدينة السادات ج.م.ع     ---   أستاذ مشارك بجامعة شقراء  المملكة العربية السعودية

الحالة الاجتماعيه

متزوج  ويعول

الــعـنوان

قسم البيوتكنولوجيا البيئية، معهد بحوث الهندسة الوراثية، جامعة مدينة السادات - مصر

الكلية التطبيقية بالقويعية - جامعة شقراء

قسم الأحياء، كلية العلوم والدراسات الإنسانية بالقويعية، جامعة شقراء - السعودية

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المؤهلات الدراسية

الدرجة العلمية

التخصص

الجهة المانحة لها

التاريخ

الدكتوراه فى العلوم

التقنية الحيوية الميكروبية

 كلية العلوم - جامعة برونشفيج – المانيا الإتحادية

2007

الماجستير في العلوم

خلية ووراثة

كلية العلوم  - جامعة المنوفية

1997

البكالوريوس

نبات وميكروبيولوجى

 كلية العلوم  - جامعة المنوفية

1990

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السجل الوظيفي

الوظيفة

جهة العمل وعنوانها

التاريخ

أستاذ

جامعة مدينة السادات

2018م – وحتى الآن

أستاذ مشارك

جامعة شقراء -

2014م – وحتى الآن تاريخه

أستاذ مشارك

جامعة مدينة السادات

2013م

أستاذ مساعد

جامعة المنوفيه

2008م

مدرس مساعد

جامعة المنوفيه

2000م

معيد

جامعة المنوفيه

1994م

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التخصص ومجالات الاهتمام

التخـصص العام

أحياء دقيقة وتقنية حيوية

التخصص الدقيق

البيوتكنولوجيا البيئية 

مـجالات الاهتمام

العلمية

General Experience:

             Bacteria isolation, purification and identification from different environmental samples

             Identification of cultured and uncultured bacteria

             DNA isolation from gram negative and gram positive bacteria and also from soil

             PCR amplification using universal or specific primers, cloning, Gene libraries, Screening of the gene libraries,    

               sequencing, sequence analysis,

             The use of SSCP-PCR and others PCR-based approaches to recognize between bacterial species at the strain

               level.

             Probes and primers design for the functional genes

             Screening and Constructing of genetically modified bacterial degraders  for the Aromatic compounds e.g.  Dioxin

              and PCBs

             Phylogenetic analysis of 16S ribosomal RNA genes either for cultured or yet uncultured microbial species from

               different environment Ecosystems

             Amplification of specific fragments of nucleic acids (DNA, RNA) using   polymerase chain reaction (PCR)

             Detection and separation of PCR products using agarose gel electrophoresis.

             Phage and phagmid library

             Fosmid library

             Overexpression of genes 

             Protein electrophoresis

             Isolation of the function fraction protein by FBLC

             Identification of the metabolites by HPLC, HPLC-Mass spectrum, and NMR

             Cloninig in gram negative and gram positive bacteria by using vector and also shuttle   vector

             Enzyme Kinetics 

             RNA isolation and RT PCR

             Gene Expression using differential display technique.

             Plastid transformation

Main research Topics:

             Bacterial degredation of the biphenyl ethers

             PCR–Single-Strand-Conformation Polymorphism (PCR-SSCP) DNA Fingerprinting for Bacterial Community

               Structures of Aromatic Hydrocarbon (AH) Contaminated Soils and New Method for Isolating (AH) Degraders

             Gene transformation in the chloroplast

             Dioxygenase enzymes and its applications for medicine in the pharmaceuticals manufacturing

             Bacterial halophiles and biodegreadation of aromatic compounds

             Tributyltin degradation

             Biodegradation of aromatic compounds and its application in bio-electrochemical systems

             Genetic characterization of antibiotic resistance and virulence factors in bacteria

Projects:

• Principle Investigator: Methods for screening dioxygenase enzymes and organisms that will catalyze the hydroxylation of a variety of aromatic substrates, and using the hydroxylated products in the applications for medicine in the pharmaceuticals manufacturing (STDF) (2009) (successfully finished 2014 )

• Principle Investigator: Plastidic expression of X- or N-containing xenobiotic aromatic compounds degradative dioxygenase for the bioremediation and production of pharmaceuticals compounds

(Joint Egyptian Japanese Scientific Cooperation (JEJSC)) (2011) (successfully finished 2014)

Supervisions

• M.Sc. Thesis with Ms. Mai Adel Hussein Abd Elmoty under title of “Analysis of the known bacterial genomes to support Environmental Applications’’ at GEBRI Menofia University (finished)

• M.Sc. Thesis with Mr. Mohamed Ibrahim under title of “Elucidate bacterial communities residing by Molecular biology methods in drinking water supply system (DWSS) " at GEBRI Menofia University (finished)

• M.Sc. Thesis with Ms. Reham Elataar under title of ‘‘Isolation and Characterization of multiple novel aerobic  polychlorinated biphenyl (PCB)-utilizing bacterial strains indigenous to contaminated soil sites’’

 at GEBRI Menofia University (finished)

• M.Sc. Thesis with Mr. Abou Baker Aly Ahmed under title of ‘‘Bacterial degradation  of  Benzene, toluene, ethylbenzene and xylenes (BTEX)  at GEBRI Sadat city University (finished)

• M.Sc. Thesis with Mr. Ahmed Awad under title of ‘‘Bacterial degradation of aromatic and haloaromatics compounds’’  at GEBRI Menofia University (Finished)

• M.Sc. Thesis with Ms. Asmaa Ahmed Aly  Title:-  “Biodegradation of aromatic compounds under extreme condition “at GEBRI Sadat city University (finished)

•M.Sc. Thesis with Ms. Somaya  El-komy Abd elmaksoud Dawah Title:-  “biodegradation of antfouling paints which pose serious detrimental impact on the human and the coastal ecosystem “at GEBRI +Sadat city University (finshed)

•M.Sc. Thesis with Ms. Seham Shaban Abd elhady Aly Title:-  Genetic characterization of antibiotic resistance and virulence factors in bacteria from Egyptian milk and dairy products”at GEBRI Sadat city University (finshed)

•M.Sc. Thesis with Ms. Esraa Elsayed Saleh,  Title “Biodegradation of aromatic compounds and its application in bio-electrochemical systems ” at GEBRI Sadat city University (finshed)

M.Sc. Thesis with Ms. Afaf  El-Sayed Mohamed Salam ,  Title “Identification and characterization of new dioxygenase genes from novel aromatic compounds degrading extremophilic bacteria”at faculty of science- Menoufia Uni.(finshed)

M.Sc. Thesis with Ms. Gehad Hassan Hamed Zanoun ,  Title “Molecular bacterial and chemical investigation of the  biodegradation of organic compound wastes using graphene oxide” ”

at faculty of science- Menoufia Uni. (finished)

Honour awards and prizes

Menofia University prize for the international publishing, 2008.

Award from Partnership & Ownership Initiative, launched by the Egyptian Ministry of Higher Education and State for Scientific research in order to promote Excellency in Higher Education and Scientific Research ´Travel Grant" up to six months

Reviewer for international journals

Reviewer for the

1-            Journal of environmental pollution  Elsevier) Impact factor 5

2-            journal Biodegradation, published by Springer. Impact factor 2.017

3-            Current trends in Biotechnology and Pharmacy  Scopus®database H Index  – 4 ( Elsevier)

الابحاث العلمية المنشورة

عنوان البحث والناشر والتاريخ

اسم الباحث (الباحثين)

Hassan HA, Dawah SE, El-Sheekh MM (2019) Monitoring the degradation capability of novel haloalkaliphilic tributyltin chloride (TBTCl) resistant bacteria from butyltin-polluted site. Rev Argent Microbiol 51:39– 46. doi.org/10.1016/j.ram.2017.12.002

Hamdy A. Hassan ,

Somya E. Dawah, Mostafa M. El-Sheekh

SugE belongs to the small multidrug resistance (SMR) protein family involved in tributyltin (TBT) biodegradation and bioremediation by alkaliphilic  Stenotrophomonas chelatiphaga HS2. International Journal of Biological Macromolecules 2018 Mar;108:1219-1226  https://doi.org/10.1016/j.ijbiomac.2017.11.02

Hamdy A. Hassan

Isolation and characterization of three novel catechol 2,3-dioxygenase from three novel haloalkaliphilic BTEX-degrading Pseudomonas strains. International Journal of Biological Macromolecules, 2018, Vol. 106, pp. 1107-1114.

Hamdy A. Hassan 

, Asmaa A. Aly

Monitoring the quality of mineral bottled water concerning to potential pathogenic bacteria and nitrate levels” Journal of Water Chemistry and Technology, 2017, Vol. 39, No. 2, pp. 115–123.

Hamdy A. Hassan,

Mohammed I. Mostfa.

Prevalence, antibiotic resistance and virulence  of Enterococcus spp. in Egyptian fresh raw milk cheese. Food Control, 2015, Food Control 50, 815-820.

Ahmed M. Hammad,

Hamdy A. Hassan,

Tadashi Shimamoto.

Culture - independent Pathogenic Bacterial Communities in Bottled Mineral Water. Journal of Renewable Energy and Sustainable Development (RESD), 2015,1(1): 197-204. DOI: http://dx.doi.org/10.21622/resd.2015.01.1.197

Hamdy a. Hassan,

Khattab, Omkolthom H., Eglal A. Ghoneimy, Abo-elnasr AA, Hamdy Abdelazeim Hassan, and Mohamed Ya Hassan. "

Role of Microorganisms in our life's as ecofrindely and replacement for chemical methods." International Journal of Pharmacy & Life Sciences, 2015, 6, no. 2.

Khatab, Omkolthom H., Muftah AA Nasib, Eglal A. Ghoneimy, A. A. Abo-Elnasr, Hamdy Abdel-Azeim Hassan, Mohamed YA Hassan, and Idress Hamad Attitalla

The role of plant hormones in balancing immune responses and increase yield production. Bulletin of Pharmaceutical and Medical Sciences (BOPAMS), 2015, 3(2): 3121-3135.

Mohamed Y. A. Hassan Omkolthom H. Khattab1, Eglal A. Ghoneimy, Abo-Elnasr .A.A, Hamdy Abdel-Azeim Hassan

Characterization of vancomycin-resistant Staphylococcus aureus in the National Liver Institute. Menoufia Medical Journal, , 2014, 27(4), p.825. 10.4103/1110-2098.149802

Ghoniem, E.M., El Hendawy, G.R., Moteleb, T.M.A., Hassan, H.A. and Khalil, H.A.E.R.

Cloning and Expression of meta-Cleavage Enzyme of BTEX Degradation Pathway from Haloalkaliphilic Pseudomonas sp. HA10. Life Science Journal, 2014, 11(5):403-411.

Hamdy A. Hassan,

Asmaa A. Aly, Mohamed E. Ebeid,

Microbiological Studies on the Wastewater Treatment and Agricultural Fertilization EJAS Journal-2014-2-2, 29-32.

Mohamed Younes Abdelfatah Hassan, Omkolthom Hassan Khatab , Eglal Abd Alla Ghoneimy ,Abo-Elnasr A.A ,Hamdy Abdel-Azeim Hassan 

2014. Investigation Of Polyphenolic Compounds, Cytotoxic And Antimicrobial Activities Of Callistemon Comboynensis Leaves. Natural product Journal, 2014, vol 10 (1): 13-16

Hamdy A. Hassan

, Amel M. Kamal, Mohamed I. Abdelhady,  Munjid Ibrahim

Cloning and Kinetic Properties of Catechol 2,3-dioxygenase from Novel Alkaliphilic BTEX-degrading Pseudomonas sp. HB01. Life Science Journal, 2014, 11(2):  353-360.

Hamdy A. Hassan

, Zain Eldein AA, Risk NMH,

Detection, Cloning, and Expression of catechol 2,3 dioxygenase Genes from Novel Polychlorinated Biphenyl (PCB) Degraders. Life Science Journal; 11(1)  1123-1132

Hamdy A. Hassan.

Identification and characterization of dioxygenase genes in new polychlorinated biphenyls degraders Life Science Journal, 2013, 10(3)  889-900

Hamdy A. Hassan,

El-Sayed A.El-Absawy, Reham M. El.attar, Nashwa MK Risk.

Monitoring the microbiological and chemical quality of bottled water during shelf life. Egypt. J. Exp. Biol. (Bot.),2013, 9(1): 27 – 34

Hamdy A. Hassan,

Mohamed I. Mostfa,  Ragaa A. Hamouda, Nashwa MK Risk.

 Detection of Pathogenic Bacterial Communities in Bottled Mineral Water by Culture -independent Molecular Technique. International conference focused on the Arab MENA region and EuroMed that entitled “Global climate change, biodiversity and sustainability: challenges and opportunities” in cooperation with University of Prince Edward Island (PEI) - Canada and Smithsonian Conservation Biology Institute - Virginia - USA, 2013 April 15 - 18,, at AASTMT - AbuKir Campus - Alexandria.

Hamdy A. Hassan. 

Detection of New Polychlorinated Biphenyl (PCB) Degraders Express Meta-cleavage Enzymes in PCB Contaminated Site. Life Science Journal, 2013, 10(1)  1123-1132

Hamdy A. Hassan.

Potential Pharmaceutical Compounds from Dioxygenase-Derived Chiral Metabolite,  Current Trends in Biotechnology and Pharmacy, 2012, 6 (4): 389-397.

Hamdy A. Hassan

Isolation and Characterization of Halophilic, Aromatic and Chloroaromatic Degrader  from Wadi El-Natrun Soda lakes, Life Science Journal, 2012 9(3): 1565-1570

Hamdy A. Hassan,

Nashwa MH Rizk, M. A. Hefnawy, and Ahmad M. Awad.

16S rRNA gene-based Bacterial Community in olychlorinated Biphenyls (PCBs) contaminated site using  PCR- Single-Strand Conformation Polymorphism (SSCP) Life Science Journal, 2012 9(3): 935-939.

Hamdy A. Hassan

Antioxidant and antimicrobial activities of Callistemon comboynensis essential oil. Free Radicals and Antioxidants, 2012, 2(1):37-41

Mohamed I. Abdelhady  and Hamdy A. H. Aly.)

Cloning and Expression of Three Extradiol Dioxygenases Type I from the Unique Dioxin Degrader Sphingomonas Wittichii RW1. J Am Sci, 2012, 8(3):40-45.

Mahmoud I. Nasr, Hamdy A.Hassan, Hanafy A.Hamza, El-Sayed A.El-Absawy and Mai A. Omar

PCR–Single-Strand-Conformation Polymorphism (PCR-SSCP) DNA Fingerprinting for Bacterial Community Structures of Aromatic Hydrocarbon (AH) Contaminated Soils and New Method for  Isolating (AH) Degraders” First International Conference on Biotechnology and Environmental Safety National research center, Cairo, Egypt, 2009, April 14-16,  P 1-13 

Hamdy A. H. Aly.

Two angular dioxygenases contribute to the metabolic versatility of dibenzofuran-degrading Rhodococcus sp. strain HA01. Appl. Environ. Microbiol., 2008, 74(12), 3812-3822

Hamdy A. H. Aly

,  N. B. Huu, V. Wray, H. Junca, and D. H. Pieper.

Molecular detection and diversity of novel diterpenoid dioxygenase DitA1 genes from proteobacterial strains and soil samples. Environ. Microbiol., 2007, 9(5), 1202–1218  

Witzig, R., Hamdy A. H. Aly,

C. Strömpl, V. Wray, H. Junca, and D. H. Pieper.