حمدي عبدالعظيم حسن علي

• Prof. Hamdy Abdel- Azeim Hassan Aly
• ORCID iD 
• https://orcid.org/0000-0002-1552-571

• Personal Data:
• Name 
• Hamdy Abdel- Azeim Hassan Aly
• Job Title:
• Professor
• Nationality: Egyptian.
• Martial status: Married + 4 children.
•   Current address:
• Department of Biological  science, Faculty of Science and Humanity Studies at Al- Quwayiyah, Shaqra University, Al-Quwayiyah 11971, Saudi Arabia
• Home address:
•   Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), Sadat city University, Sadat city –Egypt. P.O. Box 79
•  

General Experience:

• Bacteria isolation, purification and identification from different environmental samples
• Identification of cultured and uncultured bacteria
• DNA isolation from gram negative and gram positive bacteria and also from soil
• PCR amplification using universal or specific primers, cloning, Gene libraries, Screening of the gene libraries, sequencing, sequence analysis,
• The use of SSCP-PCR and others PCR-based approaches to recognize between bacterial species at the strain level.
• Probes and primers design for the functional genes
• Screening and Constructing of genetically modified bacterial degraders  for the Aromatic compounds e.g.  Dioxin and PCBs
• Phylogenetic analysis of 16S ribosomal RNA genes either for cultured or yet uncultured microbial species from different environment Ecosystems
• Amplification of specific fragments of nucleic acids (DNA, RNA) using   polymerase chain reaction (PCR)
• Detection and separation of PCR products using agarose gel electrophoresis.
• Phage and phagmid library
• Fosmid library
• Overexpression of genes 
• Protein electrophoresis
• Isolation of the function fraction protein by FBLC
• Identification of the metabolites by HPLC, HPLC-Mass spectrum, and NMR
• Cloninig in gram negative and gram positive bacteria by using vector and also shuttle vector
• Enzyme Kinetics 
• RNA isolation and RT PCR
• Gene Expression using differential display technique.
• Plastid transformation