Portulaca oleracea L. (PO), commonly known as purslane, is using as medicinal plant due to its high contents of bioactive compounds. Only few studies have been performed on the biochemical descrip-tions of P. oleracea cultivated in Egypt. Therefore, the present study aimed to determine the nutritional composition of the Egyptian PO leaves. The samples were analyzed for their proximate chemical compo-sition, minerals, vitamins, amino acids and fatty ac-ids. Results showed that, PO leaves contains 19.39% ash, 12.13% protein and 7.24% crude fiber. While the minerals content were: potassium 1283.50 mg/100 g DW., calcium 793.35 mg/100 g and magnesium 517.56 mg/100 g. Furthermore, PO leaves is rich in vitamin A (382.54 μg/100g) and 16 of the most important amino acids, 8 out of them are essential amino acids. Moreovere, PO leaves contains (47.92%) omega-3 fatty acid, twenty-seven phenolic compounds also were identified by HPLC. Data concluded that the purslane leaves excellent re-source of pharmaceutical and biochemical com-pounds that has biological impacts on human health and could be used in the nutraceutical applications.